REU RESEARCH PROJECTS
Program
Goals: The goal of this program is to introduce and educate the participants
about the basic difference between conventional Newtonian fluids and complex
fluids by examples, show them the micro-scale devices in laboratory, teach them
the tools (both experimental and analytical) for analysis of the behavior of
these fluids, and then provide them research experience in applying those tools
in independent research projects with assigned faculty members. All participants will choose
a research topic that contributes to the goals of an on-going research project
of one of the participating faculty, who will serve as the student’s advisor. The
REU students will be integrated into their advisor’s research group, typically
consisting of other undergraduate students, graduate students, post-doctoral
associates, and visiting faculty. The more experienced students and post-docs,
will provide REU students with assistance in day-to-day activities, such as
demonstrating typical laboratory procedures, and will provide feed-back on
their research activities (e.g., results, experimental design) on a regular
basis. The faculty advisor will explain the research objectives to the student
about his/her project, help the student outline a research approach, and assist
in the design of initial experiments or solution approach (including the use of
software) that will achieve those objectives. The faculty advisor will
encourage the student to become more independent with respect to research
planning, data interpretation and analysis of results while insuring that the
student makes progress toward his/her specific objectives. The research
projects will be designed to give the REU students an opportunity to apply the
principles of experimental design, data collection, and data analysis from
experiment as well as computational software that are formally introduced in
various workshops. Although the workshops and research projects are expected to
reinforce each other, most projects will not place equal emphasis on all topics
covered in the workshops. Nonetheless, all students will be exposed to various
aspects of both experiments and theory behind data collection and analysis
throughout the summer, and they will obtain a deeper understanding of the
subject matter related to the behavior of complex fluids and their applications
by combining these complementary activities. Examples of potential REU research
projects are given below.
Faculty:
1. Ramesh K. Agarwal,
William Palm Professor of Engineering, department of Mechanical & Aerospace
Engineering (analytical & computational modeling of Newtonian and complex
fluids in micro-devices, computational & experimental study of nano-fluids)
2. Da-Ren Chen,
Associate Professor, department of Mechanical & Aerospace Engineering and
Environmental Engineering
Program (nanoparticle transport in microscale systems and their formation in
chemically reactive flow systems)
3. Elliot Fried,
Professor, department of Mechanical & Aerospace Engineering
(nematic-isotropic phase transitions in micro-channels, volumetric phase
transitions in hydrogels)
4. Rohit V. Pappu,
Assistant Professor, department of Biomedical Engineering and Center for
Computational Biology
(folding and self-assembly of proteins)
5. Amy Q. Shen,
Assistant Professor, department of Mechanical & Aerospace Engineering
(transport & interfacial dynamics of complex fluids in microfluidic
devices)
6. Radhakrishna
Sureshkumar, Associate Professor, department of Chemical Engineering
(nonlinear rheological behavior of complex fluids, turbulent drag reduction)
Research Projects:
Transport and Interfacial Dynamics of Complex Fluids
Inside Micro-fluidic Devices (A. Shen)
REU Project #1: Critical Determinants of Droplet Formation for a Lipid Solution inside a Microfluidic Network
Droplet formation is a commonplace occurrence and has
been studied for hundreds of years. Despite this fact, it is, in many instances
still hard to characterize and predict. Droplets can be formed through many
different means. The system examined in the current study is that of a stream
of one fluid shearing or pinching off the flow of another fluid. The two competing
forces involved in the formation of these droplets are the shear stress, caused
by the flow of the outer or shearing fluid and capillary stress, which is
a result of the interfacial tension trying to minimize the surface area between
the two fluids. The capillary stress resists the deformation of the inner
fluid, until the point where it is overcome by the shear stress. Once the
shear stress is larger than the capillary stress, the inner flow pinches off
into droplets. For flows of a complex fluid in shear, this problem may become
increasingly complicated.
REU Project #2: Making Liquid Crystal Droplets inside Micro-fluidic
Channels
Based
on a recent paper on crystal structure in nematic emulsion with temperature
dependence and formed chemically, one undergraduate researcher will perform
the experiment to create liquid crystal droplets inside a silicone oil reservoir
inside a micro-fluidic channel with flow-focusing geometry. This study will
develop a more efficient technique to form and control liquid crystal emulsions.
The student will perform systematic studies by varying temperature, viscosity
ratio of the two phases (liquid crystal and silicon oil with different viscosities),
and ratio of flow rates of two phases to build a phase diagram for the conditions
where liquid crystal droplets can be formed.
REU Project #3: Interplay of
Application
of Micellar Fluids in Fire Fighting (R. Sureshkumar)
Polymers
and surfactant assemblies offer tremendous potential as additives to the fire
hose streams to enhance the range and throughput, to delay the jet breakup and
to produce larger drops that are less susceptible to entrainment and can
sustain evaporative losses, and to enhance the fire extinction capabilities of
the aqueous solutions by suppressing rebound from, and by improving the
wettability on hydrophobic surfaces. Presently the sole criterion for the
selection of surfactant additives used in fire fighting systems is the ability
of the surfactant to reduce the surface tension of the aqueous phase to promote
wetting and foaming based on research that reached a mature state in the 1950s.
Since then, there has been tremendous progress in our understanding of
rheology, physical chemistry and non- Newtonian fluid mechanics of polymeric
and surfactant systems, especially on the influence of additives on jet breakup
and drop formation. This understanding presents us with unique opportunities to
reevaluate the selection criteria for additives as well as to develop novel
product designs that will help enhance the present capabilities to combat
catastrophic urban fires in an efficient and safe manner. The goal of the REU project described below is to exploit
these opportunities.
REU Project #4: Experimental Investigation of the Role of Drag
Reducing Surfactants in Fire Fighting Equipment Design
Systematic
experimental studies will be conducted by an undergraduate researcher to
understand the role of solution rheology, chemical composition, and flow rate
on the reduction in pressure drop, jet breakup, and the size distribution and
rebound characteristics of the drops under conditions of turbulent flow in test
sections equipped with nozzles that simulate the single bore and fog nozzles
used in municipal fire fighting. Different designs for the application of the
additives such as the use of homogeneous solution and the use of periodic
injection to overcome polymer degradation effects will be explored.
Calorimetric studies will also be performed to evaluate the influence of the
additives on the specific heat capacity of the aqueous solutions. A
well-characterized flow system equipped with flow control valves, flow meters,
and high speed camera (1000 Hz) will be used to carry out the experiments. Two
drag reducing surfactant systems will be used. The first one is cetyltrimethyl
ammonium chloride (CTAC), a drag reducing cationic surfactant, with 2-, 3-, or
4-chloro benzoic acid (CB) as the counter ion. We have used CTAC/CB systems in
our earlier investigations to identify the role of surfactant additives on
turbulent mixing flows and have found that even at small concentrations below
the critical micelle concentration (CMC), the self assembly into rod-like
micelles can occur in the presence of flow [9]. In the proposed project, the
surfactant concentrations will be of O(103) ppm. This will reduce
surface tension of water to values of 30 dynes/cm or below required in fire
fighting applications to promote wettability. The second surfactant system that
will be selected for this study is Ethoquad T/13-50 (a C16
hydrocarbon based surfactant available from Akso chemicals) with sodium
salicylate (NaSal) that has been found to produce up to 80% drag reduction when
used in12:5 molar ratio of Ethoquad to NaSal with 2000 ppm of Ethoquad concentration.
By performing experiments by using surfactant formulations with different molar
ratios of surfactant and the counter ion, we will identify chemical
compositions that are optimal not solely based on the drag reduction percentage
but also based on the other criteria relevant to fire fighting, namely the
breakup length of the jet, drop size and drop rebound characteristics. This
project will also be used for outreach to K-12 students and for public
demonstrations.
Protein
Folding and Self-Assembly (R. Pappu)
Pappu’s
research group focuses on unfolded proteins and their role in folding,
self-assembly, post-translational modifications, and protein function. The main
goal is to understand the interplay between information encoded in amino-acid
sequences and the role of aqueous mixtures in influencing conformational
equilibria and hierarchical self-assembly processes. These interests are
pursued using tools based on a combination of molecular simulations and
theories adapted from fields such as polymer physics and the theory of liquids,
and
spectroscopic approaches pursued through collaborations with experimentalists
within and outside
In the past, undergraduate students in Pappu’s group
have focused on answering specific questions pertaining to sequence-structure
relationships and conformation-dependent hydration structure of proteins and
peptides. A brief description of two REU summer research projects is given
below.
REU Project #5:
Spectroscopic Signatures Generated by Short, Disordered Peptides
Short peptides are model systems to study local
conformational preferences in unfolded proteins. The main spectroscopic tool
used in the study of disordered peptides and unfolded proteins is ultraviolet
circular dichroism (UV-CD). A CD spectrum corresponds to an average over an
ensemble of conformations and does not allow one to extract quantitative
information regarding conformational propensities. Conversely, detailed
information is available using molecular simulation, especially using novel
methods for rapid and efficient sampling of conformational space developed in
Pappu’s lab. One possible way to connect theory and experiment would be to
predict CD spectra generated using the simulated ensemble of conformers. In
this project, the REU student will use software made available to us by
Professor Robert Woody of
REU Project # 6: Consensus Sequences of Peptides that are
Likely to Inhibit Aggregation
Genetic mutations that lead to expanded polyglutamine
tracts in specific neuronal proteins are the main cause for at least eight
different progressive inherited neurological disorders including Huntington’s
disease. Pappu’s lab is studying ways to stabilize the soluble monomeric form
of polyglutamine to prevent aggregation as a possible route to therapeutic
intervention. This is a difficult task in light of experimental evidence
suggesting that polyglutamine monomers behave like disordered random coils in
solution. However, recent work in the lab has shown that individual
polyglutamine chains are an ensemble of conformations where segments of the
chain fluctuate about left-handed, three-residue-per-turn, polyproline II (PII)
helices connected by flexible bends. Chain solubility increases when the polyglutamine
chains are in PII-like geometries. The goal is to conceive of ways
to increase the PII propensity of polyglutamine chains. This
requires the design of peptides that will bind to polyglutamine in PII-like
conformations. Numerous instances of these putative ligands are available in
protein-protein interaction domains. The undergraduate student will identify
consensus sequences for putative peptide ligands using relational database.
Molecular docking simulations will allow the prediction of binding affinities
of peptide ligands to polyglutamine peptides in different conformations.
Nanoparticle
Formation and Transport in Micro-scale Systems (Da Ren Chen)
Nanoparticles
are considered a building block in nanotechnology applications, therefore their
formation and characterization has acquired a great importance in recent years.
Nanoparticle transport in micro-scale systems and their formation in chemically
reactive flow systems provide special research challenges. Dr. Da Ren Chen has
several funded research programs in this area from NSF, NASA, and DOE. A brief
description of two REU projects is given below.
REU Project # 7: Nanoparticle Transport in Microscale Condensation
Particle Counter
Condensation
Particle Counters (CPCs) are one of the primary tools for counting particles
in sizes down to 3 nm. They play an
important role in the study of nanoparticles, which are now considered a
building block in nanotechnology applications. The operational principle of
CPCs is to grow the size of sampled particles into a larger size so that they
can be easily counted by light scattering technique. To achieve the goal, a
particle carrier gas is passed through a vapor-saturated chamber (called
saturator) and then introduced into a cooling chamber (called condenser) where
the temperature is reduced to the level such that the supersaturated vapor
condition is achieved. When particles enter the condenser, the extra vapor
quickly condenses on the particles and the size of particles is thus increased.
By the time the particles reach the condenser exit, their sizes have “matured”
to the super-micron range. Particles in the matured size range can be easily
detected with the light scattering technique using a low cost light source. Although
many applications of CPCs have been evidenced and CPCs are considered as the
standard tools for sub-micron and nanoparticle studies, the commercially
available CPCs cost more than $25K. The high cost of the instrument prevents
its use in monitoring network applications.
To overcome this problem, Dr. Chen and the researchers at NASA Glenn
have been working together to develop a micro-scale CPC using the MEMS
manufacturing technique. The use of MEMS manufacturing process reduces the
production cost of each micro-CPC to the affordable level that makes the
networking application of CPCs viable. Although the prototype device has been
tested and has provided promising results, the fundamental understanding of the
entire process is necessary for further improvement of the device. The device
is the first MEMS device, in which a two-phase flow at micro-scale is
involved. In the device, the working
fluid (alcohol-based solvent) is introduced into the micro-scale saturator by the
effect of surface tension. In the micro-scale saturator, the fluid evaporates
into the vapor. The vapor saturated environment at micro-scale is thus
established. In the condenser, the supersaturated vapor environment is set up
by the temperature control. Due to reduced temperature, the extra vapor will
condense on particles as well as on the walls of the micro-scale condenser. The
management of this liquid condensation is of importance in the device
operation. The details of liquid-vapor motion under such micro-scale
environment are largely unknown. Furthermore, the details of transport behavior
of nanometer particles in such a micro-scale two-phase flow environment remain
unexplored. A REU student will be recruited every summer to set up the
experiments for investigation of the complex two-phase fluid behavior in the
micro-scale CPC.
REU Project #8: Nanoparticle Formation in Chemically Reactive Flow
Systems
Nanoparticles
are often produced using the so called aerosol reactors. The reactors could be
in a closed system e.g. tubular furnaces, or in an open system e.g., flames. In
the laboratory setting, both systems are used to synthesize the nanoparticles.
For example, funded by NSF Nanoscale Science and Engineering Program and led by
Dr. Chen, magnetic nano- and nano-composite particles for medical applications
are produced at
Analytical,Computational,
and Experimental Study of Flow and Heat Transport in Micro-Devices & in
Nanofluids (R. Agarwal)
Fluid
flows in micro-devices, e.g. micro-sensors, micro-ducts, micro-actuators,
micro-valves, micro-pumps etc. are significantly different than those in
macroscopic devices due to their small characteristic sizes. The inertial
forces, for example tend to be quite small and surface effects tend to dominate
their behavior. Friction, electrostatic forces, and viscous effects due to the
surrounding air or liquid become increasingly important as the devices become
smaller. Agarwal and his group have developed analytical models and
computational codes to study the gas flows in micro-devices using Navier-Stokes
and Burnett equations with slip flow. They have also developed
Lattice-Boltzmann codes for studying these flows. More recently, they have also
been studying the problem of convective heat transport in nanofluids. The
concept of nanofluids has been advanced by Choi, who showed substantial
augmentation of heat transported in suspensions of copper or aluminum
nanoparticles in water and other liquids. A nanofluid is more or less a uniform
dispersion of very small particles held in suspension by Brownian motion. This
allows us to consider the solid-liquid mixture in a nanofluid as a composite
fluid with properties like viscosity and thermal conductivity dependent upon
the properties, concentration and size of the suspended particles. Thus we
study the convective heat transport in a nanofluid experimentally as well as by
solving a set of continuum equations for a composite fluid.
REU Project #9: Gas Flows in a
Micro-Devices
For
gas flows in micro-fluidic configurations, the Knudsen layer close to the wall
can comprise a substantial portion of the entire flow field and has major
effect on quantities such as mass flow rate through micro-devices. Additionally
the Maxwell’s slip boundary condition needs to be modified for curved
boundaries and moving surfaces. We will include the latest developments in
treating the slip velocity and temperature conditions on curved/moving walls
including the wall- function approach in our Navier-Stokes and Burnett codes.
This project will be coordinated with the experimental work being performed in
Prof. Amy Shen’s lab. The principal investigator will work closely with the
student to complete these tasks. Other project of PI in this area involves the
application of magnetic field on flow in micro-channels. These flows occur in
magnetic thin films and other electromagnetic micro-scale devices. It has been
shown that the integration of magnetic field in micro-fluidic MEMS design can
enhance the performance of these devices in a variety of applications. This
project will involve the development of a simple analytical model for the MHD
slip flow in a micro-channel and use of an existing Lattice-Boltzmann based
simulation code.
REU Project # 10: Natural Convection in a 2-D Cavity Filled with a
Nanofluid:
The
convective heat transport due to buoyancy in a 2-D cavity filled with a
nanofluid will be studied by numerical simulation. UDF functions in the CFD
software “FLUENT” will be modified for this purpose. A small scale experiment
will also be designed by modifying the existing experimental set up in the heat
transfer laboratory. Computations will be compared with the experimental data
to validate the modeling of a nanofluid as a composite fluid and to investigate
the augmentation of heat transport in a nanofluid. The undergraduate student
will work closely work with the PI in designing the experiment and taking the
data.
Nematic-Isotropic
Phase Transitions (E. Fried)
When
quenched from a high-temperature isotropic phase to a low-temperature nematic
phase, a liquid crystal undergoes a first-order phase transition. Such
transitions proceed via the nucleation, growth, and coalescence of droplets.
Experiments involving free- and directional-growth show that the nematic-isotropic-phase-interfaces
exhibit a host of interesting morphological instabilities, which are manifested
by the formation of dendrites and periodic cellular patterns resembling those that
occur during crystal growth.
REU Project # 11: Simulations of Nematic-Isotropic Phase Transitions
in Micro-channels
These
simulations will be based on a sharp-interface theory developed recently by
Cermelli, Fried and Gurtin. This theory provides a generalization of the
Ericksen–Leslie theory for uniaxial nematics which (a) allows for phase
transitions, (b) models a nematic-isotropic interface as a sharp surface across
which bulk fields may suffer discontinuities, and (c) accounts for localized
interactions between phases by endowing the interface with excess properties. The
theory yields evolution equations for the bulk phases and for the interface. In
addition to the standard equations enforcing momentum and energy balance on the
interface, the theory involves an ancillary equation that enforces configurational
momentum balance on the interface. The configurational momentum balance
provides a generalization of the Gibbs–Thomson relation (familiar from sharp-interface
theories of solidification) appropriate to the description of nematic-isotropic
phase transitions. The simulation effort will require the extension of a computer
code by Dolbow, Fried and Ji, recently developed for the study of volumetric
phase transitions in hydrogels. This code combines the eXtended Finite-Element
Method (XFEM) and the Level-Set Method (LSM) to provide a robust, accurate, and
efficient tool for the simulation of phase transitions. The investigator will
work closely with the students to complete that extension. The simulations will
be tied closely with experimental observations performed in the laboratory of
Prof. Amy Shen.
Workshops: During a typical 10-week REU
summer program, four workshops and a series of seminars on research projects
will be held. The duration of each workshop will be about a day long
(9:00a.m-12:00p.m,
Workshop #1: Introduction to Complex Fluids (Shen, Sureshkumar,
Fried)
This workshop will introduce the students to basic
difference between complex fluids and normal fluids by examples. It will
describe some of their applications in everyday life. The fundamental mathematical relations that characterize
them will be introduced. The experiments and software employed in the study of
these fluids at
Workshop #2: Introduction to Microfluidics (Agarwal, Shen)
It will introduce the students to fabrication of
micro-devices in the lab. It will introduce them to the measurement techniques
and instrumentation. It will describe the special physics that occurs even in
normal Newtonian fluids due to micro-scale. The analytical, computational, and
experimental tools to characterize the fluid behavior at micro-scale will also
be introduced.
Workshop #3: Behavior of Complex Fluids in Micro-devices including
Protein Folding & Self Assembly (Shen, Fried, Pappu)
The students will be introduced to the special
dynamics of complex fluids and interfacial phenomenon that occurs in complex
fluids at micro-scale, in contrast to normal Newtonian fluids.
They will also be introduced to the problems of
protein folding and self-assembly. Both the experimental set up and software
used in these studies will be explained.
Workshop #4: Nanoparticle Formation & Transport in Microscale
Systems including the Behavior of Nanofluids (Chen, Agarwal)
The physics of nanoparticles formation and their
transport will be introduced. The instrumentation and software used at
The basic concepts behind nanofluids will be
introduced. The software and the experimental apparatus employed in the study
of nanofluids will be described.
Workshop #5: Ethics Workshop
An
ethics workshop will be conducted. The workshop will include lectures by ethics
professors at WU, presentations by outside speakers from industry and academia,
case studies, group discussions among students and faculty on concepts and
skills for resolution of ethical issues, etc. The goal of the workshop will be
to make students aware of the importance of ethical conduct in everyday life,
be it in personal or professional environment.
Additional
Activities (Industry Visits, Social Activities, Seminars etc.):
In addition to research and participation in
workshops, a variety of additional activities will be included in the program
to broaden the students’ exposure to practical applications of their research
and to build their technical communications skills. An orientation meeting will
be held on the first day of the program to introduce all the participating
students and faculty to each other, to familiarize the students with Washington
University facilities (e.g., the libraries, computing facilities, laboratories,
gym etc.), and to discuss laboratory safety. The exposure to practical applications of their research in complex
fluids, micro-fluidics, and nanotechnology will be accomplished through visits
to local engineering companies, e.g. Boeing, Monsanto, MEMC, Applied Materials,
Cross-link Polymers etc. Finally, the students’ communications skills will
be developed through submission of biweekly reports and oral presentations on
their research projects. All REU students will be required to submit biweekly
written reports and make several oral presentations on the progress of their
research. All written reports will be
reviewed by at least two participating faculty—the student’s research advisor
and one other—who will provide the student with written feedback designed to
help the student improve their report-writing skills. In addition, each student
will review and provide feedback on the progress reports submitted by another
fellow student. Making students review the reports of other students is
expected to teach them to be more critical when they edit their own work. All
students will also be required to submit a final report summarizing their
research goals, methods, results, and conclusions. All biweekly and final
reports will be posted on the REU Program’s web site. An archive of these
reports will be accessible to prospective REU students in subsequent years.
Oral presentations will be made during weekly meetings that will be attended by
all the participating students and faculty, as well as others who may be
interested. In general, each REU student will make three 20-minute
presentations on their research during the course of the 10-week program. During the first week of the program, the
weekly meeting will focus on the fundamentals of oral presentations, including
examples of good and bad habits. One faculty member (not the student’s advisor)
and one REU student will be assigned to provide written feedback to each
student on his/her presentation, especially focusing on organization, quality
of visual aids, and whether the results that were presented provided adequate
support for the conclusions.
At the conclusion of the 10-week program, all
students will make formal final presentations at the
which is normally attended by a large number of
undergraduate students and faculty in science and engineering. During 10 weeks of the program, several organized
social events (e.g., introductory barbeque, group lunches/dinners, evening at
sporting events etc.) will be held periodically to facilitate the development
of lasting collegial relationships among the students and the faculty.